JRC GMO-Amplicons | EURL GMFF

Record ID:	BK010737.1
Database:	nt	Go to original record
Description:	TPA: Nicotiana knightiana chloroplast, complete genome.
Taxon:	Nicotiana knightiana
Original size:	155963 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	49319	49890	+	572 bp

Amplicon:

572 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acttactaag tgatcacttt caaattcaga gaaaccctgg aattaacaaa
101   aatgggcaat cctgagccaa atcctgtttt ccgaaaacaa acaaaggttc
151   agaaaaaaaa ggataggtgc agagactcaa tggaagctat tctaacaaat
201   ggagttaaat gcgttggtag aggaatcttt acatcgaaac ttcagaaaga
251   aaaagaatga agtgaaggat aaacgtatat acatacgtat tgaatactat
301   atcaaatgat taatgatgac ccgaatccat attttttcta taaaaaatag
351   aagagttggt gtgaatcgat tctacattga agaaagaatc gaatattcat
401   tgatcaaatc attcactcca tagtctgaca gatcttttga agaactgatt
451   aatcggacga gaataaagat agagtcccgt tctacatgtc aataccggca
501   acaatgaaat ttatagtaag aggaaaatcc gtcgacttta aaaatcgtga
551   gggttcaagt ccctctatcc cc

View other 2 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.58e-08
primerB	100.0	20 bp	0	0	572	553	2.58e-08

Match details