JRC GMO-Amplicons | EURL GMFF

Record ID:	LR537510.1
Database:	nt	Go to original record
Description:	Brachypodium hybridum genome assembly, organelle: plastid:chloroplast
Taxon:	Brachypodium hybridum
Original size:	136328 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46609	47220	+	612 bp

Amplicon:

612 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tagtatggaa
51    acctgctaag tgttaacttc caaattcaga gaaaccctgg aattaaaaaa
101   gggcaatcct gagccaaatc cgtgttttga gaaaacaagg ggttctcgaa
151   ctagaatcca aaggaaaagg ataggtgcag agactcaatg gaagctgttc
201   taacgaatcg agttaattag gttgttttgg tagtggaact cctttaaaat
251   tagagaaaga agggatttat acatctaata aacacgtata gatactaaca
301   tagcaaacga ttaatcacag aatctatagt ataacatagg ttctttattc
351   ttttttagaa tgaaattagg aaggattatg aaataaaaat tgaataaatt
401   ttttagaatt attgtgaatc cattctaatt gaatcttgag taatcaaatc
451   cttcaattta aagtactcga gatcttttaa aaagtggatt aatcggacga
501   ggacaaagag agagtcccat tctacatgtc aatactgaca acaatgaaat
551   ttcgagtaaa aggaaaatcc gtcgacttta taagttgtga gggttcaagt
601   ccctctatcc cc

View other 7 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.76e-08
primerB	100.0	20 bp	0	0	612	593	2.76e-08

Match details