JRC GMO-Amplicons | EURL GMFF

Record ID:	MT381997.1
Database:	nt	Go to original record
Description:	Luffa aegyptiaca chloroplast, complete genome.
Taxon:	Luffa aegyptiaca
Original size:	157275 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	49501	50093	+	593 bp

Amplicon:

593 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc ctttttccga aaacaaaaaa aaggggtagg
151   tgcagagact caatggaagc tgttctaaca aatggagttg actacgttgc
201   gttagtaaag gaatgaatcc ttctatcgaa actccagaaa agatgaaaga
251   taaacgtatt acgtacggaa atactatttg attaatgaca acccgaatct
301   ctattttttt ttatatatat ataaatataa aaaatgatat ataaaaatga
351   ccgaattgtt atgaatcgat tccaatatct ccaagttgaa aaaagaatcg
401   aatattcatt gatcaaatca tttactccat catacatagt ctgatagatc
451   ttttgaagaa ctgattgatc agatgcgaat agaataaaga tagagtccca
501   ttctacatgt caataccgac aaaaatgaaa tttatagtaa gaggaaaatc
551   cgtcgacttt aaaaatcgtg agggttcaag tccctctatc ccc

View other 3 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.67e-08
primerB	100.0	20 bp	0	0	593	574	2.67e-08

Match details