JRC GMO-Amplicons | EURL GMFF

Record ID:	MT381996.1
Database:	nt	Go to original record
Description:	Luffa acutangula chloroplast, complete genome.
Taxon:	Luffa acutangula
Original size:	157202 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	49426	50019	+	594 bp

Amplicon:

594 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc ctttttccga aaacaaaaaa aaggggtagg
151   tgcagagact caatggaagc tgttctaaca aatggagttg actacgttgc
201   gttagtaaag gaatgaatcc ttctatcgaa actccagaaa agatgaaaga
251   taaacgtatt acgtactgaa atactatttg attaatgaca acccgaatct
301   ctattttttt tttatatata tataaatata aaaaatgata tatcaaaatg
351   accgaattgt tatgaatcga ttccaatatc tccaagttga aaaaagaatc
401   gaatattcat tgatcaaatc atttactcca tcatacatag tctgatagat
451   cttttgaaga actgattgat cagatgcgaa tagaataaag atagagtccc
501   attctacatg tcaataccga caaaaatgaa atttatagta agaggaaaat
551   ccgtcgactt taaaaatcgt gagggttcaa gtccctctat cccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.68e-08
primerB	100.0	20 bp	0	0	594	575	2.68e-08

Match details