JRC GMO-Amplicons | EURL GMFF

Record ID:	V00178.1
Database:	nt	Go to original record
Description:	Maize gene for tRNA-Leu(UAA) (contains an intron).
Taxon:	Zea mays
Original size:	640 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	91	622	+	532 bp

Amplicon:

532 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aatgaaaaat
101   gggcaatcct gagccaaatc ccttttttga aaaacaagtg gttctcaaac
151   tagaacccaa aggaaaagga taggtgcaga gactcaatgg aagctgttct
201   aacgaatcga agtaataacg attaatcaca gaacccatat tataatatag
251   gttctttatt ttatttttag aatgaaatta ggaatgatta tgaaatagaa
301   aattcataat ttttttttag aattattgtg aatctattcc aatcaaatat
351   tgagtaatca aatccttcaa ttcattgttt tcgagatctt ttaattttaa
401   aaagtggatt aatcggacga ggataaagag agagtcccat tctacatgtc
451   aatactgaca acaatgaaat ttctagtaaa aggaaaatcc gtcgacttta
501   taagtcgtga gggttcaagt ccctctatcc cc

View other 10 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.39e-08
primerB	100.0	20 bp	0	0	532	513	2.39e-08

Match details