JRC GMO-Amplicons | EURL GMFF

Record ID:	AF148733.1
Database:	nt	Go to original record
Description:	Georgeantha hexandra tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Georgeantha hexandra
Original size:	1018 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	600	+	600 bp

Amplicon:

600 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tgttaacttc caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc cctattttga gaaaacaaac gtctataagg
151   ataggtgcag agactcaatg gaagctgttc taacgaataa agttgattat
201   gttgtgttgg tagtgagact ccctctaaat tagagactgg aggggcttat
251   atatctaata cacgtgccta tatactgatg attaatcatg gaacctgtat
301   gagaatatat attatttctt cttttttcta aaattcagaa tggttatgaa
351   ataggaaatt ccacattttt tttataatta ctctgaatca atttccatcg
401   aagttgaaag aagaatcgaa tatcgagtaa taaaatcatt cattccatag
451   ttttctcaat ttttgaaaaa aacggattaa tcggacgaga ataaagatag
501   agtcccattc tacatgtcaa tatcgacaac aatgaaattt ctagtaaaag
551   gaaaatccgt cgaattttta aatcgtgagg gttcaagtcc ctctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.70e-08
primerB	100.0	20 bp	0	0	600	581	2.70e-08

Match details