JRC GMO-Amplicons | EURL GMFF

Record ID:	AY145343.1
Database:	nt	Go to original record
Description:	Saururus cernuus trnT-trnL intergenic spacer, trnL gene and trnL-trnF intergenic spacer sequence.
Taxon:	Saururus cernuus
Original size:	1819 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	891	1464	+	574 bp

Amplicon:

574 bp

1     cgaaatcggt agacgctacg gacttnnnga ttggattgag ccttggtatg
51    gaaacctact aagtggtaac ttccaaattc agagaaaccc tggaatgaaa
101   aatgggcaat cctgagccaa atcctatttt cataaaacaa gagttcagaa
151   agcgagaatc aaataaaaaa aatggatagg tgcagagact caatggaagc
201   tgttctaacg aatggggtta cctttattaa ttacacattc attggtagag
251   gaatccttct atcgaaactc cagaaagaat gaccctagat acgtactgaa
301   atatcaaaga ttaatcacga cccgaatcct tatttttttt ttcagaataa
351   ttctaaaatt gttctgaatc gattccagaa ggaagaatcg aatattcagt
401   gatcaaggca ttcactccgg agtatgatgg atcttttgaa gaactgatta
451   atcggacgag aataaagata gagtcccatt ctacatgtca atactgacag
501   caatgaaatt tatagtaaga ggannnaaat ccgtcgactt tagaaatcgt
551   gagggttcaa gtccctctat cccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.58e-08
primerB	100.0	20 bp	0	0	574	555	2.58e-08

Match details