JRC GMO-Amplicons | EURL GMFF

Record ID:	AF502039.1
Database:	nt	Go to original record
Description:	Sideritis montana tRNA-Leu (trnL) gene, partial sequence; chloroplast gene for chloroplast product.
Taxon:	Sideritis montana
Original size:	563 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	562	+	562 bp

Amplicon:

562 bp

1     cgaaatcggt agacgctacg sacttaatga taattaaatt gagccttatt
51    gtgggaaacc tactaagtga tgactttcaa attcagagaa accccggaat
101   taatcaaaat gggcgatcct gatccaaatc ctgttttttc aaaacaaagg
151   ttcaaaaaac caaataaagg ataggtgcag agactcaacg gaagctgttc
201   taacaaatgg agttactgcg ttggtagagg aatccttttt acggaaactt
251   cagaaaggat gaaggataaa cgtatctatc gaatactata tcaaatgatt
301   aatgatggcc cgaatctgta ttttttaata tgaaaaatag aagaattggt
351   gtgaattgat tctatattga agaaaaaatc gaatattcat tcatcaaacc
401   actcactcca tagtccggta gatcttttaa agacctgatt aatcggacga
451   gaataaagat agagtcccat tctacatgtc aatactggca acaatgaaat
501   ttatagtaag aggaaaatcc gtcgacttta aaaatcgtga gggttcaagt
551   ccctctatcc cc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.53e-08
primerB	100.0	20 bp	0	0	562	543	2.53e-08

Match details