JRC GMO-Amplicons | EURL GMFF

Record ID:	AF502031.1
Database:	nt	Go to original record
Description:	Phyllostegia glabra tRNA-Leu (trnL) gene, partial sequence; chloroplast gene for chloroplast product.
Taxon:	Phyllostegia glabra
Original size:	564 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	563	+	563 bp

Amplicon:

563 bp

1     cgaaatcggt agacgctacg gacttaatta taattaaatt gagccttagt
51    gtgggaaacc tactaagtga taactttcaa attcagagaa accccggaat
101   taatcaaaat gggcgatcct gatccaaatc ctgttttttc aaaacaatgg
151   ttcaaaaaac caaataaagg ataggtgcag agactcaacg gaagctgttc
201   taacaaatgg agttgactgc gttggtagag gaatcctttc tacggaaact
251   tcagaaagga tgaaggataa acgtatctat cgaatactat atcaaatgat
301   taatgatggt ccgaatccgt atttttgaat atgaaaaata gaagaattgg
351   tgtgaattga ttctatattg aagaaaaaat cgaatattca ttcatcaaat
401   cattcactcc atagtccgat agatctttta aagacctgat taatcggacg
451   agaataaaga tagagtccca ttctacatgt caataccggc aacaatgaaa
501   tttatagtaa gaggaaaatc cgtcgacttt aaaaatcgtg agggttcaag
551   tccctctatc ccc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.53e-08
primerB	100.0	20 bp	0	0	563	544	2.53e-08

Match details