JRC GMO-Amplicons | EURL GMFF

Record ID:	AY944539.1
Database:	nt	Go to original record
Description:	Mimozyganthus carinatus voucher R. Fortunato 7575 (BAB, BH) tRNA-Leu gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe gene, partial sequence; chloroplast.
Taxon:	Mimozyganthus carinatus
Original size:	1101 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	2	622	+	621 bp

Amplicon:

621 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctaccaag tgataacttt caaattcaga gaaaccctgg aattaacaat
101   gggcaatcct gagccaaatc ctgttttccg aaaaccaaga agagttcaga
151   aagggagaat aaaaataaaa aaaggatagg tgcagagact caacggaagc
201   tgttctaaca aatggagttg acgacatttc gtttagttag taaagatttc
251   gtttcgttac taaagagatc cttccatcga aactccagaa aagaaaggat
301   caaggatgag catacgtata cgtactgaaa tactatttca attgattaga
351   ccagacagac caaaaatctc tatttttgaa tttttatgac aaataaaaga
401   tgtgaatcga ttccaagttg aagaaagaaa agaatcgaat attaattgat
451   caaatcattc actccatgat agtctgatag atcttttgaa gaactgatta
501   atcagacgag aataaagata gagtcccatt ctacatgtca ataccgacaa
551   caatgaaatt tatagtaaga ggaaaatccg tcgactttag aaatcgtgag
601   ggttcaagtc cctctatccc c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.80e-08
primerB	100.0	20 bp	0	0	621	602	2.80e-08

Match details