JRC GMO-Amplicons | EURL GMFF

Record ID:	AY944537.1
Database:	nt	Go to original record
Description:	Parkinsonia praecox voucher R. Fortunato 7566 (BAB, BH) tRNA-Leu gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe gene, partial sequence; chloroplast.
Taxon:	Parkinsonia praecox
Original size:	1067 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	2	591	+	590 bp

Amplicon:

590 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctaccaag tgataacttt caaattcaga gaaaccctgg aattaacaat
101   gggcaatcct gagccaaatc ctgttttccg aaaaccaaga agagttcaga
151   aggggagaat aaaaaaagga taggtgcaga gactcaacgg aagctgttct
201   aacaaatgga gttgacaaca tttcgtttcg ttagtaaagg aatccttcca
251   tcgaaactcc agaaaagaaa ggatcaagga tgaacatacg tatacgtact
301   gaaatactat ttcaattgat tagaccagac agaccccaaa tctctatttt
351   ttcattttta tgacaaatga aagatgtgaa tcgattccaa gttgaagaaa
401   gaatcgaata ttcattgatc aaatcattca ctccatcata gtctgataga
451   tcttttcaag aactgattaa tcggacgaga ataaagatag agtcccattc
501   tacatgtcaa taccgacagc aatgaaattt atagtaagag gaaaatccgt
551   cgactttaga aatcgtgagg gttcaagtcc ctctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.66e-08
primerB	100.0	20 bp	0	0	590	571	2.66e-08

Match details