JRC GMO-Amplicons | EURL GMFF

Record ID:	AY944536.1
Database:	nt	Go to original record
Description:	Cercidium andicola voucher R. Fortunato 7638 (BAB, BH) tRNA-Leu gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe gene, partial sequence; chloroplast.
Taxon:	Parkinsonia andicola
Original size:	1072 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	2	597	+	596 bp

Amplicon:

596 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctaccaag tgagaacttt caaattcaga gaaaccctgg aattaacaat
101   gggcaatcct gagccaaatc ctgttttccg aaaaccaaga agagttcaga
151   aggggagaat aaaaaaagga taggtgcaga gactcaacgg aagctgttct
201   aacaaatgga gttgacaaca tttcgtttcg ttagtaaagg aatccttcca
251   tcgaaactcc agaaaagaaa ggatcaagga tgaacatacg tatacgtata
301   cgtactgaaa tactatttca attgattaga ccagacagac cccaaatctc
351   tattttttca tttttatgac aaatgaaaga tgtgaatcga ttccaagttg
401   aagaaagaat cgaatattca ttgatcaaat cattcactcc atcatagtct
451   gatagatctt ttcaagaact gattaatcgg acgagaataa agatagagtc
501   ccattctaca tgtcaatacc gacaacaatg aaatttatag taagaggaaa
551   atccgtcgac tttagaaatc gtgagggttc aagtccctct atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.68e-08
primerB	100.0	20 bp	0	0	596	577	2.68e-08

Match details