JRC GMO-Amplicons | EURL GMFF

Record ID:	DQ018178.1
Database:	nt	Go to original record
Description:	Cremastosperma oblongum tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Cremastosperma oblongum
Original size:	963 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	576	+	576 bp

Amplicon:

576 bp

1     cgaaatcggt agacgctacg gacttgattg gattgagcct tggtatggaa
51    acctastaag tgagamcttc caraytcaka saaaccctgg aattaaaaat
101   gggcaatcmt gagccaaatc ctgtgttcag maracaaggt tcagaaagcg
151   agaatcaata wcagaaaaag gataggtgca gagactcaac ggaagctgtt
201   ctaacaaata gagttgactg cattggtagg ggaattcttt ccttctaccc
251   aaacgacaga aaggatgacc ttgtatacgt acgtatacat actgaaacat
301   caaacgatta atcacgactc gaatcagtat ttttttatat gaaaaatttc
351   agaatagaag gattgtgaat tgattccgag ttgaaggaag aatcgaatat
401   tcagagatca aatcattcat tcccgagtct aatagatctt ttgaagaact
451   gattaatcgg acgagaataa agatagagtc ccattctaca tgtnnnnnnn
501   nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
551   nnnnnggttc aagtccctct atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.59e-08
primerB	100.0	20 bp	0	0	576	557	2.59e-08

Match details