JRC GMO-Amplicons | EURL GMFF

Record ID:	AF440738.1
Database:	nt	Go to original record
Description:	Amorphophallus smithsonianus tRNA-Leu (trnL) gene, partial sequence; chloroplast gene for chloroplast product.
Taxon:	Amorphophallus smithsonianus
Original size:	562 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	2	561	+	560 bp

Amplicon:

560 bp

1     cgaaatcggt agacgctacg ggctttgatt gcattgagcc ttggtatgga
51    aacctactaa gtggtaactt ccaaactcag agaaaccctg gaataaaaaa
101   tgggcaatcc tgagccaaat ccttgttttg agaaaaaagg ataggtgcag
151   agactcaatg gaagctgttc taacaaatgg agttgattgc attgcgttgg
201   tagctggaat tcctctttct atctaaatta cagaaaagat aaccctatat
251   acctaatacg cacgtatata tactgacata tcaaacgatt aatcacgacc
301   caaatccata ataattattt atataattat aataaattca aaattttatg
351   aaaaattaaa gagttattgc gaatccattc taaattgaag taagagtcga
401   atattcagtg atcaaatcat ttgaaaaaaa aaatgattaa tcggacgaga
451   ataaagagag agtcccattc tacatgtcaa taccgacaac aatgaaattt
501   atagtaagag gaaaatccgt cgactttaga aatcgtgagg gttcaagtcc
551   ctctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.52e-08
primerB	100.0	20 bp	0	0	560	541	2.52e-08

Match details