JRC GMO-Amplicons | EURL GMFF

Record ID:	AF387480.1
Database:	nt	Go to original record
Description:	Anchomanes difformis tRNA-Leu (trnL) gene, partial sequence; chloroplast gene for chloroplast product.
Taxon:	Anchomanes difformis
Original size:	591 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	2	590	+	589 bp

Amplicon:

589 bp

1     cgaaatcggt agacgctacg gacttgattg cattgagcct tggtatggaa
51    acctactaag tggtaacttc caaactcaga gaaaccctgg aataaaaaat
101   gggcaatcct gagccaaatc cttgttttgt tttgagaaaa aaggataggt
151   gcagagactc aatggaagct gttctaacga atggagttga ttgcattgcg
201   ttggtagctg gaattcttct ttctatctaa attacagaaa agataaccct
251   atatacctaa tacgcacgta tatatactga catatcaaac gattaatcac
301   gacccaaatc cataattttt atttatataa tgaattccaa attttatgaa
351   aaatttaaga gttattgtga atccattcca aattgaagta agagtggaat
401   attcattgat caaatcatta actccagagt ctgattaatc ttttgaaaaa
451   tgaaaaaaaa aatgattaat cggacgagaa taaagagaga gtcccattct
501   acatgtcaat accgacaaca atgaaattta tagtaagagg aaaatccgtc
551   gactttagaa atcgtgaggg ttcaagtccc tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.65e-08
primerB	100.0	20 bp	0	0	589	570	2.65e-08

Match details