JRC GMO-Amplicons | EURL GMFF

Record ID:	AF387477.1
Database:	nt	Go to original record
Description:	Amorphophallus zenkeri tRNA-Leu (trnL) gene, partial sequence; chloroplast gene for chloroplast product.
Taxon:	Amorphophallus zenkeri
Original size:	576 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	2	575	+	574 bp

Amplicon:

574 bp

1     cgaaatcggt agacgctacg gacttgattg cattgagcct tggtatggaa
51    acctactaag tggtaacttc caaactcaga gaaaccctgg aataaaaaat
101   gggcaatcct gagccaaatc cttgttttga gaaaaaagga taggtgcaga
151   gactcaacgg aagctgttct aacaaatgga gttgattgca ttgcgttggt
201   agctggaatt attctttcta tctaaattct aaattacgga aaagataacc
251   ctatatacct aatacgcacg tatatatact gacatatcaa acgattaatc
301   acgacccaaa tccataataa ttatttatat aattataata taataaattc
351   aaaattttat gaaaaattaa agagttattg cgaatccatt ctaaattgaa
401   gtaagagtcg aatattcagt gatcaaatca tttgaaaaaa aaaaaaatga
451   ttaatcggac gagaataaag agagagtccc attctacatg tcaataccga
501   caacaatgaa atttatagta agaggaaaat ccgtcgactt tagaaatcgt
551   gagggttcaa gtccctctat cccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.58e-08
primerB	100.0	20 bp	0	0	574	555	2.58e-08

Match details