JRC GMO-Amplicons | EURL GMFF

Record ID:	AF387476.1
Database:	nt	Go to original record
Description:	Amorphophallus yunnanensis tRNA-Leu (trnL) gene, partial sequence; chloroplast gene for chloroplast product.
Taxon:	Amorphophallus yunnanensis
Original size:	567 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	2	566	+	565 bp

Amplicon:

565 bp

1     cgaaatcggt agacgctacg gacttgattg cattgagcct tggtatggaa
51    acctactaag tggtaacttc caaactcaga gaaaccctgg aataaaaaat
101   gggcaatcct gagccaaatc cttgttttga gaaaaaagga taggtgcaga
151   gactcaatgg aagctgttct aacaaatgga gttgattgca ttgcgttggt
201   agctggaatt cttctttcta tctaaattac agaaaggata accctatata
251   cctaatacgc acgtatatat actgacatat caaaccaaac gattaatcac
301   gacccaaatc cataataatt atttatataa ttataataaa ttcaaaattt
351   tatgaaaaat taaagagtta ttgcgaatcc attctaaatt gaagtaagag
401   tcgaatattc agtgatcaaa tcatttgaaa aaaaaaaatg attaatcgga
451   cgagaataaa gagagagtcc cattctacat gtcaataccg acaacaatga
501   aatttatagt aagaggaaaa tccgtcgact ttagaaatcg tgagggttca
551   agtccctcta tcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.54e-08
primerB	100.0	20 bp	0	0	565	546	2.54e-08

Match details