JRC GMO-Amplicons | EURL GMFF

Record ID:	AF387468.1
Database:	nt	Go to original record
Description:	Amorphophallus pygmaeus tRNA-Leu (trnL) gene, partial sequence; chloroplast gene for chloroplast product.
Taxon:	Amorphophallus pygmaeus
Original size:	599 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	2	598	+	597 bp

Amplicon:

597 bp

1     cgaaatcggt agacgctacg gacttgattg cattgagcct tggtatggaa
51    acctactaag tggtaacttc caaactcaga gaaaccctgg aataaaaaat
101   gggcaatcct gagccaaatc cttgttttga gaaaaaagga taggtgcaga
151   gactcaatgg aagctgttct aacaaatgga gttgattgca ttgcgttggt
201   agctggaatt cttctttcta tctaaattac agaaaagata accctatata
251   cctaatacgc acgtatattg acatatcaaa cgattaatca cgacccaaat
301   ccataataat tatttatata aataataatt atttatataa tatataatta
351   taatatatat aattataata aattcaaaat tttatgaaaa attaaagagt
401   tattgcgaat ccattctaaa ttgaagtaag agtcgaatat tcagtgatca
451   aatcatttga aaaaaaaaaa tgattaatcg gacgagaata aagagagagt
501   cccattctac atgtcaatac cgacaacaat gaaatttata gtaagaggaa
551   aatccgtcga ctttagaaat cgtgagggtt caagtccctc tatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.69e-08
primerB	100.0	20 bp	0	0	597	578	2.69e-08

Match details