JRC GMO-Amplicons | EURL GMFF

Record ID:	AF387466.1
Database:	nt	Go to original record
Description:	Amorphophallus pingbianensis tRNA-Leu (trnL) gene, partial sequence; chloroplast gene for chloroplast product.
Taxon:	Amorphophallus pingbianensis
Original size:	577 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	2	576	+	575 bp

Amplicon:

575 bp

1     cgaaatcggt agacgctacg gacttgattg cattgagcct tggtatggaa
51    acctactaag tggtaacttc caaactcaga gaaaccctgg aataaaaaat
101   gggcaatcct gagccaaatc cttgttttga gaaaaaagga taggtgcaga
151   gactcaatgg aagctgttct aacaaatgga gttgattgca ttgcgttggt
201   agctggaatt cttctttcta tctaaattac agaaaggata accctatata
251   cctaatacgc acgtatatat actgacatat caaacgatta atcacgaccc
301   aaatccataa taattattta tataattata atttatataa ttataataaa
351   ttcaaaattt tatgaaaaat taaagagtta ttgcgaatcc attctaaatt
401   gaagtaagag tcgaatattc agtgatcaaa tcatttgaaa aaaaaaaatg
451   attaatcgga cgagaataaa gagagagtcc cattctacat gtcaataccg
501   acaacaatga aatttatagt aagaggaaaa tccgtcgact ttagaaatcg
551   tgagggttca agtccctcta tcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.59e-08
primerB	100.0	20 bp	0	0	575	556	2.59e-08

Match details