JRC GMO-Amplicons | EURL GMFF

Record ID:	AF387465.1
Database:	nt	Go to original record
Description:	Amorphophallus palawanensis tRNA-Leu (trnL) gene, partial sequence; chloroplast gene for chloroplast product.
Taxon:	Amorphophallus palawanensis
Original size:	595 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	2	594	+	593 bp

Amplicon:

593 bp

1     cgaaatcggt agacgctacg gacttgattg cattgagcct tggtatggaa
51    acctactaag tggtaacttc caaactcaga gaaaccctgg aataaaaaat
101   gggcaatcct gagccaaatc cttgttttga gaaaaaagga taggtgcaga
151   gactcaatgg aagctgttct aacaaatgga gttgattgca ttgcgttggt
201   agctggaatt cctctttcta tctaaattac agaaaagata accctatata
251   cctaatacgc acgtatatat actgacatat caaacgatta atcacgaccc
301   aaatccataa tcataataat tatttatata ataattattt atataatatt
351   tatataatta taataaattc aaaattttat gaaaaattaa agagttattg
401   cgaatccatt ctaaattaaa gtaagagtcg aatattcagt gatcaaatca
451   tttgaaaaaa aaaaaatgat taatcggacg agaataaaga gagagtccca
501   ttctacatgt caataccgac aacaatgaaa tttatagtaa gaggaaaatc
551   cgtcgacttt ataaatcgtg agggttcaag tccctctatc ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.67e-08
primerB	100.0	20 bp	0	0	593	574	2.67e-08

Match details