JRC GMO-Amplicons | EURL GMFF

Record ID:	AF387464.1
Database:	nt	Go to original record
Description:	Amorphophallus paeoniifolius tRNA-Leu (trnL) gene, partial sequence; chloroplast gene for chloroplast product.
Taxon:	Amorphophallus paeoniifolius
Original size:	563 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	2	562	+	561 bp

Amplicon:

561 bp

1     cgaaatcggt agacgctacg gacttgattg cattgagcct tggtatggaa
51    acctactaag tggtaacttc caaactcaga gaaaccctgg aataaaaaat
101   gggcaatcct gagccaaatc cttgttttga gaaaaaagga taggtgcaga
151   gactcaatgg aagctgttct aacaaatgga gttgattgca ttgcgtcggt
201   agctggaatt cttctttcta tctaaattac agaaaagata atcctatata
251   cctaatacac acgtatatat actgacatat caaacgatta atcacgaccc
301   aaatccataa taattattta tataattata aaaaattcaa aattttatga
351   aaaattaaag agttattgcg aatccattct aaattgaagt aagagtcgaa
401   tattcagtga tcaaatcatt tgaaaaaaaa aaaatgatta agcggacgag
451   aataaagaga gagtcccatt ctacatgtca ataccgacaa caatgaaatt
501   tatagtaaga ggaaaatccg tcgactttaa aaatcgtgag ggttcaagtc
551   cctctatccc c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.53e-08
primerB	100.0	20 bp	0	0	561	542	2.53e-08

Match details