JRC GMO-Amplicons | EURL GMFF

Record ID:	AF387461.1
Database:	nt	Go to original record
Description:	Amorphophallus napiger tRNA-Leu (trnL) gene, partial sequence; chloroplast gene for chloroplast product.
Taxon:	Amorphophallus napiger
Original size:	562 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	2	561	+	560 bp

Amplicon:

560 bp

1     cgaaatcggt agacgctacg gacttgattg cattgagcct tggtatggaa
51    acctactaag tggtaacttc caaactcaga gaaaccctgg aataaaaaat
101   gggcaatcct gagccaaatc cttgttttga gaaaaaagga taggtgcaga
151   gactcaatgg aagctgttct aacaaatgga gttgattgca ttgcgttggt
201   agctggaatt cttctttcta tctaaattac agaaaagata accctatata
251   cctaatacgc acgtatatat actgacatat caaacgatta atcacgaccc
301   aaatccataa taattattta tataattata ataaattcaa aattttatga
351   aaaattaaag agttattgcg aatccattct aaattgaagt aagagtcgaa
401   tattcagtga tcaaatcatt tgaaaaaaaa aaatgattaa tcggacgaga
451   ataaagagag agtcccattc tacatgtcaa taccgacaac aatgaaattt
501   atagtaagag gaaaatccgt cgactttaga aatcgtgagg gttcaagtcc
551   ctctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.52e-08
primerB	100.0	20 bp	0	0	560	541	2.52e-08

Match details