JRC GMO-Amplicons | EURL GMFF

Record ID:	AF387458.1
Database:	nt	Go to original record
Description:	Amorphophallus angolensis tRNA-Leu (trnL) gene, partial sequence; chloroplast gene for chloroplast product.
Taxon:	Amorphophallus angolensis
Original size:	593 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	2	592	+	591 bp

Amplicon:

591 bp

1     cgaaatcggt agacgctacg gacttgattg cattgagcct tggtatggaa
51    acctactaag tggtaacttc caaactcaga gaaaccctgg aataaaaaat
101   gggcaatcct gagccaaatc cttgttttga gaaaaaagga taggtgcaga
151   gactcaacgg aagctgttct aacaaatgga gttgattgca ttgcgttggt
201   agctggaatt cttctttcta tctaaattac ggaaaagata accctatata
251   cctaatacgc acgtatatat actgacatat caaacgatta atcacgaccc
301   aaatccataa taataattta tataataatt tatataatta taattataat
351   aaattattat aataaattca aaattttatg aaaaattaaa gagttattgc
401   gaatccattc taaattgaag taagagtcga atattcagtg atcaaatcat
451   ttgaaaaaaa aaaatgatta atcggacgag aataaagaga gagtcccatt
501   ctacatgtca ataccgacaa caatgaaatt tatagtaaga ggaaaatccg
551   tcgactttag aaatcgtgag ggttcaagtc cctctatccc c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.66e-08
primerB	100.0	20 bp	0	0	591	572	2.66e-08

Match details