JRC GMO-Amplicons | EURL GMFF

Record ID:	AF387454.1
Database:	nt	Go to original record
Description:	Amorphophallus lambii tRNA-Leu (trnL) gene, partial sequence; chloroplast gene for chloroplast product.
Taxon:	Amorphophallus lambii
Original size:	569 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	2	568	+	567 bp

Amplicon:

567 bp

1     cgaaatcggt agacgctacg gacttgattg cattgagcct tggtatggaa
51    acctactaag tggtaacttc caaactcaga gaaaccctgg aataaaaaat
101   gggcaatcct gagccaaatc cttgttttga gaaaaaagga taggtgcaga
151   gactcaatgg aagctgttct aacaaatgga gttgattgca ttgcgttggt
201   agctggaatt cctctttcta tctaaattac agaaaagata accctatata
251   cctaatacgc acgtatatat actgacatat caaacgatta atcacgaccc
301   aaatccataa taattattta tataattata ataaataata aattcaaaat
351   tttatgaaaa attaaagagt tattgcgaat ccattctaaa ttgaagtaag
401   agtcgaatat tcagtgatca aatcatttga aaaaaaaaaa tgattaatcg
451   gacgagaata aagagagagt cccattctac atgtcaatac cgacaacaat
501   gaaatttata gtaagaggaa aatccgtcga ctttagaaat cgtgagggtt
551   caagtccctc tatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.55e-08
primerB	100.0	20 bp	0	0	567	548	2.55e-08

Match details