JRC GMO-Amplicons | EURL GMFF

Record ID:	AF387449.1
Database:	nt	Go to original record
Description:	Amorphophallus hirsutus tRNA-Leu (trnL) gene, partial sequence; chloroplast gene for chloroplast product.
Taxon:	Amorphophallus hirsutus
Original size:	583 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	2	582	+	581 bp

Amplicon:

581 bp

1     cgaaatcggt agacgctacg gacttgattg cattgagcct tggtatggaa
51    acctactaag tggtaacttc caaactcaga gaaaccctgg aataaaaaat
101   gggcaatcct gagccaaatc cttgttttga gaaaaaaaaa aaggataggt
151   gcagagactc aatggaagct gttctaacaa atggagttga ttgcattgcg
201   ttggtagctg gaattcttct ttctatctaa attacagaaa agataaccct
251   atatacccct atatacctaa tctaatacgc acgtaaatct aatacgcacg
301   tatatatact ggcatatcaa acgattaatc acgacccaaa tccataatta
351   ttatttaatt attattattt atataatttt atataattat aataaattca
401   aaatttgaag taagagtcga atattcagtg atcaaatcat ttgaaaaaaa
451   aaaatgatta atcggacgag aataaagaga gagtcccatt ctacatgtca
501   ataccgacaa caatgaaatt tatagtaaga ggaaaatccg tcgactttag
551   aaatcgtgag ggttcaagtc cctctatccc c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.62e-08
primerB	100.0	20 bp	0	0	581	562	2.62e-08

Match details