JRC GMO-Amplicons | EURL GMFF

Record ID:	AF387448.1
Database:	nt	Go to original record
Description:	Amorphophallus henryi tRNA-Leu (trnL) gene, partial sequence; chloroplast gene for chloroplast product.
Taxon:	Amorphophallus henryi
Original size:	572 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	2	571	+	570 bp

Amplicon:

570 bp

1     cgaaatcggt agacgctacg gacttgattg cattgagcct tggtatggaa
51    acctactaag tggtaacttc caaactcaga gaaaccctgg aataaaaaat
101   gggcaatcct gagccaaatc cttgttttga gaaaaaagga taggtgcaga
151   gactcaatgg aagctgttct aacaaatgga gttgattgca ttgcgttggt
201   agctggaatt cttctttcta tctaaattac agaaaggata accctatata
251   cctaatacgc acgtatatat actgaactga catatcaaac gattaatcac
301   gacccaaatc cataataatt atttatataa ttataataaa ttcaaaattt
351   tatgaaaaat taaattaaag agttattgcg aatacattct aaattgaagt
401   aagagtcgaa tattcagtga tcaaatcatt tgaaaaaaaa aaatgattaa
451   tcggacgaga ataaagagag agtcccattc tacatgtcaa taccgacaac
501   aatgaaattt atagtaagag gaaaatccgt cgactttaga aatcgtgagg
551   gttcaagtcc ctctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.57e-08
primerB	100.0	20 bp	0	0	570	551	2.57e-08

Match details