JRC GMO-Amplicons | EURL GMFF

Record ID:	AF387445.1
Database:	nt	Go to original record
Description:	Amorphophallus eburneus tRNA-Leu (trnL) gene, partial sequence; chloroplast gene for chloroplast product.
Taxon:	Amorphophallus eburneus
Original size:	578 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	2	577	+	576 bp

Amplicon:

576 bp

1     cgaaatcggt agacgctacg gacttgattg cattgagcct tggtatggaa
51    acctactaag tggtaacttt caaactcaga gaaaccctgg aataaaaaat
101   gggcaatcct gagccaaatc cttgttttga gaaaaaagga taggtgcaga
151   gactcaatgg aagctgttct aacaaatgga gttgattaca ttgcgttggt
201   agctggaatt cctctttcta tctaaattac agaaaagata accctatata
251   cctaatacgc acatatatat actgacatat caaacgatta atcacgaccc
301   aaatccataa tcataataat tatttatata atatttatat aattataata
351   aattcaaaat tttatgaaaa attaaagagt tattgcgaat ccattctaaa
401   ttaaagtaag agtcgaatat tcagtgatca aatcatttga aaaaataaat
451   gattaatcgg acgagaataa agagagagtc ccattctaca tgtcaatacc
501   gacaacaatg aaatttatag taagaggaaa atccgtcgac tttagaaatc
551   gtgagggttc aagtccctct atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.59e-08
primerB	100.0	20 bp	0	0	576	557	2.59e-08

Match details