JRC GMO-Amplicons | EURL GMFF

Record ID:	AF387443.1
Database:	nt	Go to original record
Description:	Amorphophallus decus-silvae tRNA-Leu (trnL) gene, partial sequence; chloroplast gene for chloroplast product.
Taxon:	Amorphophallus decus-silvae
Original size:	584 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	2	583	+	582 bp

Amplicon:

582 bp

1     cgaaatcggt agacgctacg gacttgattg cattgagcct tggtatggaa
51    acctactaag tggtaacttc caaactcaga gaaaccctgg aataaaaaat
101   gggcaatcct gagccaaatc cttgttttga gaaaaaagga taggtgcaga
151   gactcaatgg aagctgttct aacaaatgga gttgattgca ttgcgttggt
201   agctggaatt cctctttcta tctaaattac agaaaagata accctatata
251   cctaatacgc acgtatatat actgacatat caaacgatta atcacgaccc
301   aaatccataa taattattta tataattata ataaattttt atataattat
351   aataaattca aaattttatg aaaaattaaa gagttattgc gaatccattc
401   taaattgaag taagagtcga atattcagtg atcaaatcat ttgaaaaaaa
451   aaaaatgatt aatcggacga gaataaagag agagtcccat tctacatgtc
501   aataccgaca acaatgaaat ttatagtaag aggaaaatcc gtcgacttta
551   gaaatcgtga gggttcaagt ccctctatcc cc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.62e-08
primerB	100.0	20 bp	0	0	582	563	2.62e-08

Match details