JRC GMO-Amplicons | EURL GMFF

Record ID:	AF387437.1
Database:	nt	Go to original record
Description:	Amorphophallus beccarii tRNA-Leu (trnL) gene, partial sequence; chloroplast gene for chloroplast product.
Taxon:	Amorphophallus beccarii
Original size:	556 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	2	555	+	554 bp

Amplicon:

554 bp

1     cgaaatcggt agacgctacg gacttgattg cattgagcct tggtatggaa
51    acctactaag tggtaacttc caaactcaga gaaaccctgg aataaaaaat
101   gggcaatcct gagccaaatc cttgttttga gaaaaaagga taggtgcaga
151   gactcaatgg aagctgttct aacaaatgga gttgattgca ttgcgttggt
201   agctggaatt cctctttcta tctaaattac agaaaagata accctatata
251   cctaatacgc acgtatatat actgacatat caaacgatta atcacgaccc
301   aaatccatat ttatataatt ataataaatt caaaatttta tgaaaaatta
351   aagagttatt gcgaatccat tctaaattga agtaagagtc gaatattcag
401   tgatcaaatc atttgaaaaa aaaaaaatga ttaatcggac gagaataaag
451   agagagtccc attctacatg tcaataccga caacaatgaa atttatagta
501   agaggaaaat ccgtcgactt tagaaatcgt gagggttcaa gtccctctat
551   cccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.49e-08
primerB	100.0	20 bp	0	0	554	535	2.49e-08

Match details