JRC GMO-Amplicons | EURL GMFF

Record ID:	AB235047.1
Database:	nt	Go to original record
Description:	Rhoicissus digitata chloroplast DNA, trnL-trnF IGS, partial sequence.
Taxon:	Rhoicissus digitata
Original size:	1013 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	593	+	593 bp

Amplicon:

593 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct taatatggaa
51    acctactaag tgataacttt caaattcagg gaaaccctgg aataaaaaat
101   ggggcaatcc tgagccaaat cctgttttcc gaaaacaacc aagggttcag
151   aaaacgataa taaaaaaagg ataggtgcag agactcaatg gaagctgttc
201   taacaaaaac aaatggagtt gactatgttg cgttgttaaa ggaatccttc
251   catcaaaatt ccagaaccag aaaggatgaa ggataaacct atatatatag
301   gtactgaaag attatatcaa atgattaatg acggctcgaa tctgtatttt
351   ttgatatgaa aaatgaaaga attattgtaa atcgattcca agttgaagaa
401   agaaaagaat cgaatattca ttgatcaaat cattcactcc atagtccgat
451   agatcttttg aagaactgat taatcggacg agaataaaga tagagtccca
501   ttctacatgt caataccgac aacaatgaaa tttatagtaa gaggaaaatc
551   cgtcgacttt aaaaatcgtg agggttcaag tccctctatc ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.67e-08
primerB	100.0	20 bp	0	0	593	574	2.67e-08

Match details