JRC GMO-Amplicons | EURL GMFF

Record ID:	AB235028.1
Database:	nt	Go to original record
Description:	Cyphostemma simulans chloroplast DNA, trnL-trnF IGS, partial sequence.
Taxon:	Cyphostemma simulans
Original size:	871 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	576	+	576 bp

Amplicon:

576 bp

1     cgaaatcggt agacgctacg gacttaagtg aattgagcct taatatggaa
51    acctactaag tgataacttt caaattcagg gaaaccctgg aataaaaaat
101   gggcaatcct gagccaaatc ccgttttccg aaaaccagaa aacgctaata
151   acaaaaggat aggtgcagag actcgatgga agctgttcta acaaaaacaa
201   atggagttga ctatgttccg ttgttaaacg aatccttcca ttaaaactcc
251   agaaccagaa aggatgaagg ataacccgat atacataggt actgaaatat
301   tatatcaaat gattaatgat ggctcgaatc tgtatttttt gatatgaaaa
351   atgaaagaat tcttgtaaat cgattccaag aaagaaaaag aagcgaatat
401   tcattgatca aatcattcac tccatagtcc gatagatctt ttgaagaact
451   cattaatcgg acgagaataa agagagagtc ccattctaca tgtcaagacc
501   gacaacaatg aaatttatag taagaggaaa atccgtcgac tttaaaaatc
551   gtgagggttc aagtccctct atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.59e-08
primerB	100.0	20 bp	0	0	576	557	2.59e-08

Match details