JRC GMO-Amplicons | EURL GMFF

Record ID:	AB235025.1
Database:	nt	Go to original record
Description:	Cyphostemma bainesii chloroplast DNA, trnL-trnF IGS, partial sequence.
Taxon:	Cyphostemma bainesii
Original size:	875 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	575	+	575 bp

Amplicon:

575 bp

1     cgaaatcggt agacgctacg gacttaagtg aattgagcct taatatggaa
51    acctactaag tgataacttt caaattcagg gaaaccctgg aataaaaaat
101   gggcaatcct gagccaaatc ccgttttccg aaaaccagaa aacgctaata
151   aaaaaaggat aggtgcagag actcgacgga agctgttcta acaaaaacaa
201   atggagttga ctatgttccg ttgttaaacg aatccttcca ttaaaactcc
251   agaaccagaa aggatgaagg ataacccgat atacataggt actgaaatat
301   tatatcaaat gattaatgat ggctcgaatc tgtatttttt gatatgaaaa
351   atgaaagaat tcttgtaaat cgattccaag aaagaaaaga agcgaatatt
401   cattgatcaa atcattcact ccatagtccg atagatcttt tgaagaactc
451   attaatcgga cgagaataaa gagagagtcc cattctacat gtcaagaccg
501   acaacaatga aatttatagt aagaggaaaa tccgtcgact ttaaaaatcg
551   tgagggttca agtccctcta tcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.59e-08
primerB	100.0	20 bp	0	0	575	556	2.59e-08

Match details