JRC GMO-Amplicons | EURL GMFF

Record ID:	AB235024.1
Database:	nt	Go to original record
Description:	Cissus sp. 6903 chloroplast DNA, trnL-trnF IGS, isolate: 6903, partial sequence.
Taxon:	Cissus sp. 6903
Original size:	1033 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	2	600	+	599 bp

Amplicon:

599 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct taatatggaa
51    acctactaag tgataacttt caaattcagg gaaaccctgg aataaaaaat
101   gggcaatcct gagccaaatc ccgttttccg aaaacgacca agggttcaga
151   aaactataat aaaaaaggat aggtgcagag actcaatgga agctattcta
201   acaaaaacaa atggagttga ctatgttgcg ttgttaaacg aatccttcca
251   tcaaaactcc agaaccagaa aggatgaagg ataaacctat atacataggt
301   attgaaatat tatatcaaat gattaatgat atcaaatgat taatgatggc
351   tcgaatctgt attttttgat atgaaaaatg aaagaattgt tgtaaataga
401   ttacaaagaa aagaatcgaa tattcattgc tcaaatcatt cactccatag
451   tccgataaat cttttgaaga actgattaat cggacgagaa taaagataga
501   gtcccattct acatgtcaat accgacaaga atgaaattta tagtaagagg
551   aaaatccgtc gactttaaaa atcgtgaggg ttcaagtccc tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.70e-08
primerB	100.0	20 bp	0	0	599	580	2.70e-08

Match details