JRC GMO-Amplicons | EURL GMFF

Record ID:	AB235020.1
Database:	nt	Go to original record
Description:	Cissus sp. 6873 chloroplast DNA, trnL-trnF IGS, isolate: 6873, partial sequence.
Taxon:	Cissus sp. 6873
Original size:	1003 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	2	588	+	587 bp

Amplicon:

587 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct taatatggaa
51    acctactaag tgataacttt caaattcagg gaaaccctgg aataaaaaat
101   gggcaatcct gagccaaatc ccgttttccg aaaacaacca agggttcaga
151   aaactataat ataataaaaa aggataggtg cagagactca atggaagcta
201   ttctaacaaa aacaaatgga gttgactatg ttgcgttgtt aaacgaatcc
251   ttccatcaaa actccagaac ccgaaaggat gaaggataaa cctatataca
301   taggtattga aatattatat caaatgatta atgatggctc gaatctgtat
351   tttttgatat gaaaaatgaa agaattgttg taaatagatt acaaagaaaa
401   gaatcgaata ttcattgctc aaatcattca ctccatagtc cgataaatct
451   tttgaagaac tgattaatcg gacgagaata aagatagagt cccattctac
501   atgtcaatac cgacaagaat gaaatttata gtaagaggaa aatccgtcga
551   ctttaaaaat cgtgagggtt caagtccctc tatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.64e-08
primerB	100.0	20 bp	0	0	587	568	2.64e-08

Match details