JRC GMO-Amplicons | EURL GMFF

Record ID:	AB235016.1
Database:	nt	Go to original record
Description:	Cissus rostrata chloroplast DNA, trnL-trnF IGS, isolate: 7501, partial sequence.
Taxon:	Cissus rostrata
Original size:	1031 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	586	+	586 bp

Amplicon:

586 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct taatatggaa
51    acctactaag tgagaacttt caaattcagg gaaaccctgg aataaaaaat
101   gggcaatcct gagccaaatc ccgttttccg aaaacaacca ggggttcaga
151   aaactatata taataaaaaa ggataggtgc agagactcaa cggaagctat
201   tctaacaaaa acaaatggag ttgactatgt tgcgttgtta aacgaatcct
251   tccatcaaaa ctccagaact agaaaggatg aaggataacc ctatatacat
301   aggtagtgaa atattatatc aaatgattaa tgatggctcg aatctgtatt
351   ttttgatatg aaaaatgaaa gaattgttgt aaatagatta caaagaaaag
401   aatcgaatat tcattgctca aatcattcac tccatagtcc gataaatctt
451   ttgaagaact gattaatcgg acgagaataa agatagagtc ccattctaca
501   tgtcaatacc gacaagaatg aaatttatag taagaggaaa atccgtcgac
551   tttaaaaatc gtgagggttc aagtccctct atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.64e-08
primerB	100.0	20 bp	0	0	586	567	2.64e-08

Match details