JRC GMO-Amplicons | EURL GMFF

Record ID:	AB235013.1
Database:	nt	Go to original record
Description:	Cissus incisa chloroplast DNA, trnL-trnF IGS, isolate: 6671, partial sequence.
Taxon:	Cissus incisa
Original size:	1012 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	2	578	+	577 bp

Amplicon:

577 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct taatatggaa
51    acctactaag tgataacttt caaattcagg gaaaccctgg aataaaaaat
101   gggcaatcct gagccaaatc ccgttttccg aaaacaacca agggttcaga
151   aaactataat aaaaaaggat aggtgcagag actcaatgga agctattcta
201   acaaaaacaa atggagttga ctatgttgcg ttgttaaacg aatccttcca
251   tcaaaactcc agaacccgaa aggatgaagg ataaacctat atacatattg
301   aaatattata tcaaatgatt aatgatggct cgaatctgta ttttttgata
351   tgaaaaatga aagaattgtt gtaaatagat tacaaagaaa agaatcgaat
401   attcattgct caaatcattc actccatagt ccgataaatc ttttgaagaa
451   ctgattaatc ggacgagaat aaagatagag tcccattcta catgtcaata
501   ccgacaagaa tgaaatttat agtaagagga aaatccgtcg acttaaaaat
551   cggagnggtt caagtccctc tatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.60e-08
primerB	100.0	20 bp	0	0	577	558	2.60e-08

Match details