JRC GMO-Amplicons | EURL GMFF

Record ID:	AB235006.1
Database:	nt	Go to original record
Description:	Cayratia pseudotrifolia chloroplast DNA, trnL-trnF IGS, isolate: 8085, partial sequence.
Taxon:	none
Original size:	1006 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	2	579	+	578 bp

Amplicon:

578 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct taatatggaa
51    acctactaag tgataacttt caaattcagg gaaaccctgg aataaaaaat
101   gggcaatcct gagccaaatc ctgttttccg aaaaccagaa aacgctaata
151   aaaaaaggat aggtgcagag actcaatgga agctgttcta acaaaaacaa
201   atggagttga ctatgactat gttacgttgt taaacgaatc cttccatcaa
251   aactccagaa ccagaaagga tgaagggtaa acctagatac atagtgaaat
301   atcaaatgat taatgatggc tcgaatcttt attttttgat atgaaaaatg
351   aaagaattgt tgtaaatcga ttccaagttg aagaaagaaa agaatcgaat
401   attcattgat caaatcattc actccatagt ccgatagatc ttttgaagaa
451   ctgattaatc agacgagaat aaagagagag tcccattcta catgtcaata
501   ccgacaacaa tgaaatttat agtaagagga aaatccgtcg actttaaaaa
551   tcgtgagggt tcaagtccct ctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.60e-08
primerB	100.0	20 bp	0	0	578	559	2.60e-08

Match details