JRC GMO-Amplicons | EURL GMFF

Record ID:	AB234992.1
Database:	nt	Go to original record
Description:	Ampelopsis brevipedunculata chloroplast DNA, trnL-trnF IGS, isolate: 6674, partial sequence.
Taxon:	Ampelopsis glandulosa var. brevipedunculata
Original size:	1031 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	2	593	+	592 bp

Amplicon:

592 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct taatatggaa
51    acctactaag tgataacttt caaattcagg gaaaccctgg aataaaaaat
101   gggcaatcct gagccaaatc ctgttttccg aaaacaacca agggttcaga
151   aaacgataat aaaaaaagga taggtgcaga gactcaatgg aagctgttct
201   aacaaaaaca aatggagttg actatgttgt gttgttaaag gaatccttcc
251   atcaaaactc cagaaccaga aaggatgaag gataaaccta tatacatagg
301   tactgaaata ttatatcaaa tgattaatga cggctcgaat ctgtattttt
351   tgatatgaaa aatgaaagaa ttgttgtaac tcgattccaa gttgaagaaa
401   gaaaagaatc gaatattcat tgatcaaatc attcactcca tagtccgata
451   gatcttttga agaactgatt aatcggacga gaataaagat agagtcccat
501   tctacatgtc aataccgaca acaatgaaat ttatagtaag aggaaaatcc
551   gtcgacttta aaaatcgtga gggttcaagt ccctctatcc cc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.67e-08
primerB	100.0	20 bp	0	0	592	573	2.67e-08

Match details