Match details

Record ID: X65195.2
Database: nt Go to original record
Description: Streptomyces viridochromogenes strain Tue494 phosphinothricin tripeptide biosynthetic gene cluster: ORFB1, ORFB2, ORF1, phsB gene, ORFM, phsC gene, pmi gene, adhP gene, ORF3, pgdP gene, ppm gene, ppd gene, ORFx, pgmP gene, cpps gene, cppm gene, aldP gene, pmet gene, pms gene, phsA gene, pat gene, dea gene, the1 gene, the2 gene, trs gene, prpA gene, ORFE1, ORFE2 and ORFE3 (partial)
Taxon: Streptomyces viridochromogenes
Original size: 37823 bp

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Method: QL-ELE-00-014 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of phosphinothricin N-acetyltransferase gene (Grohmann et al., 2009).
Target:bar

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
2 2 30020 30079 + 60 bp

Amplicon: 60 bp
1     acgagcacgg tcaacttccg tacggagccg cagactccgc aggagtggat
51    cgacgacctg  

View other 43 matches with an identical amplicon


Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 94.7 19 bp 1 0 1 19 4.93e-07


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.