JRC GMO-Amplicons | EURL GMFF

Record ID:	AF506838.1
Database:	nt	Go to original record
Description:	Taxus cuspidata chloroplast tRNA-Leu (trnL) gene, complete sequence; and trnL-trnF intergenic spacer, partial sequence.
Taxon:	Taxus cuspidata
Original size:	821 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	522	+	522 bp

Amplicon:

522 bp

1     cgaaatcggt agacgctacg gacttaatag aattgagcct tggtatggaa
51    acttactaag tgatagcttc caaatacagg ggaaccctgg aatattttga
101   atgggcaatc ctgatccaaa tccgtattat aggaacaata attttatttt
151   ctagaaaagg gataggtgca gagactcaac ggaagatatt ctaacgactt
201   aatatcattt gaatttgaac caatattcta tctacagagt gtagtatgtt
251   attgaaaact ttgaaagtgt tctgtatcat cgttaaaact tgtttcaacg
301   attagaactt gagttgttct aggcttgcct agcttaatga atacttaatt
351   aaagtaattc aattaagaaa taaatagaat ttattccatt tttgaattat
401   tggacgagga taaagataga gtccaattct acatgtaaat gccaacaaca
451   acaatgcaaa ttgcagtagt cggaaaatcc gttggtttta taaaccgtga
501   gggttcaagt ccctctatcc cc

View other 4 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.35e-08
primerB	100.0	20 bp	0	0	522	503	2.35e-08

Match details