JRC GMO-Amplicons | EURL GMFF

Record ID:	AF278509.1
Database:	nt	Go to original record
Description:	Plathymenia reticulata trnL gene and trnL-trnF intergenic spacer, partial sequences; chloroplast genes for chloroplast products.
Taxon:	Plathymenia reticulata
Original size:	1041 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	586	+	586 bp

Amplicon:

586 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctaccaag tgataacttt caaattcaga gaaaccctgg aattaacaat
101   gggcaatcct gagccaaatc ctgttttccg aaaaccaaga agagttcaga
151   aagggagaat ccaaaaagga taggtgcaga gactcaacgg aagctgttct
201   aacaaatgga gttgacgaca tttcgtttcg ttagtaaaga aatctttcca
251   tcgaaactcc aaaggatcaa ggatgagcat acgtataccg tactgaaata
301   ctatttcaat tgattagacc agacagaccc aaaatctcga tttttgaata
351   tttatatgac aaatgaaaga tgtgaatcga ttccaagttg aagaaagaat
401   cgaatattaa ttgatcaaat cattcactcc atgatagtct aatagatctt
451   ttgaagaact gattaatcag acgagaataa agatagagtc ccattctaca
501   tgtcaatacc gacaacaatg aaatttatag taagaggaaa atccgtcgac
551   tttagaaatc gtgagggttc aagtccctct atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.64e-08
primerB	100.0	20 bp	0	0	586	567	2.64e-08

Match details