JRC GMO-Amplicons | EURL GMFF

Record ID:	AF278497.1
Database:	nt	Go to original record
Description:	Stryphnodendron guianense trnL gene and trnL-trnF intergenic spacer, partial sequences; chloroplast genes for chloroplast products.
Taxon:	Stryphnodendron guianense
Original size:	1089 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	611	+	611 bp

Amplicon:

611 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctaccaag tgataacttt caaattcaga gaaaccctgg aattaacaat
101   gggcaatcct gagccaaatc ctgttttccg aaaaccaaga agagttcaga
151   aggggagaat aaaaaaagga taggtgcaga gactcaacgg aaactgttct
201   aacaaatgga gttgacgaca tttcgtttcg ttagtaaaga tttcgtttcg
251   ttagtaaaga gatccttcca tcgaaactcc agaaaagaaa ggatcaagga
301   tgagcatacc gtataccgta ctgaaatact atttcaattg attagaccag
351   acagacccaa aatctctatt ttttaatatt tatatgacaa ataaaagatg
401   tgaatcgatt ctaagttgaa gaaagaatcg aatattaatt gatcaaattc
451   actccatgat agtctgatct atttttggaa gaacttatta atcagacgag
501   aataaagata gagtcccatt ctacatgtca atactgacaa caatgaaatt
551   tatagtaaga ggaaaatccg tcgactttag aaatcgtgag ggttcaagtc
601   cctctatccc c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.75e-08
primerB	100.0	20 bp	0	0	611	592	2.75e-08

Match details