JRC GMO-Amplicons | EURL GMFF

Record ID:	AF278484.1
Database:	nt	Go to original record
Description:	Elephantorrhiza elephantina trnL gene and trnL-trnF intergenic spacer, partial sequences; chloroplast genes for chloroplast products.
Taxon:	Elephantorrhiza elephantina
Original size:	1080 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	607	+	607 bp

Amplicon:

607 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctaccaag tgataacttt caaattcaga gaaaccctgg aattaacaat
101   gggcaatcct gagccaaatc ctgttttccg aaaaccaaga agagttcaga
151   aagggagaat aaaaaaagga taggtgcaga gactcaacgg aagctgttct
201   aacaaatgga gttgacgaca tttcgtttcg ttagttcgtt agtaaaggca
251   tccttccatc gaaactccag gaaagaaaaa agaaaggatc agggatgagc
301   atacgtatac gtactgaaat actatttcaa ttgattagac cagaccgacc
351   ccaaatccct atttttgaat atttatatga caaatgaaag atgtgaatcg
401   attccaagtt gaagaaagaa tcgaatattc attgatcaaa tcattcactc
451   catcatagtc tgatagatct tttgaagaac tgattaatca gacgagaata
501   aagatagagt cccattctac atgtcaatac cgacaacaat gaaatttata
551   gtaagaggaa aatccgtcga ctttagaaat cgtgagggtt caagtccctc
601   tatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.73e-08
primerB	100.0	20 bp	0	0	607	588	2.73e-08

Match details