JRC GMO-Amplicons | EURL GMFF

Record ID:	AF278481.1
Database:	nt	Go to original record
Description:	Anadenanthera colubrina trnL gene and trnL-trnF intergenic spacer, partial sequences; chloroplast genes for chloroplast products.
Taxon:	Anadenanthera colubrina
Original size:	1090 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	616	+	616 bp

Amplicon:

616 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctaccaag tgataacttt caaattcaga gaaaccctgg aattaacaat
101   gggcaatcct gagccaaatc ctgtttttcg aaaaccaaga agagttcaga
151   aagggagaat aaggataggt gcagagactc aacggaagct gttctaacaa
201   atggagttga cgacatttcg tttcgttagt aaagatttcg tttcgttagt
251   aaagagatcc ttccatcgaa actccagaaa agaaaggatc aaggatgagc
301   atacgtatac cgtactgaaa tactatttct atttcaattg attagaccag
351   acagacccaa aatctctatt ttttaatatt tatatgacaa ataaaagatg
401   tgaatcgatt ccaagttgaa gaaagaatcg aatattctat taattgatca
451   aattcactcc atgatagtct gatagatctt ttgaagaact gattaatcag
501   acgagaataa agatagagtc ccattctaca tgtcaatacc gacaacaatg
551   aaatttatag taagaggaaa atccgtcgac ttttgaaatc gtgagggttc
601   aagtccctct atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.78e-08
primerB	100.0	20 bp	0	0	616	597	2.78e-08

Match details