JRC GMO-Amplicons | EURL GMFF

Record ID:	AF327601.1
Database:	nt	Go to original record
Description:	Ulmus glabra tRNA-Leu (trnL) gene, intron; chloroplast gene for chloroplast product.
Taxon:	Ulmus glabra
Original size:	598 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	598	+	598 bp

Amplicon:

598 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctaccaag tgataacttt caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc ctgttttatg aaaataaggg ttcataaagc
151   gataataaaa aaggataggt gcagagactc aatggaagct gttctaacaa
201   atggagttgg ctgtattact ttagtaagta aaggaatcct tccatcgaaa
251   ctccagaaag agaaaggatg aagaatcaac ctatatacct actgaaatac
301   tatcgtcaaa tgattaataa ctgattaata acgacatgaa tccgtattaa
351   taatatattt atattaaaaa tgaaagaatt gttgtgaatc aatttcaaat
401   tgaaaaacga atcgaatatt catcgatcaa atcatttact ccatcaaaat
451   ctgatagatc ttttgaagaa ttgattaatc ggacgagaat aaagatagag
501   tcccattcta catgtcaata tcgacaacaa tgaaatttag agtaagagga
551   aaatccgtcg actttagaaa tcgtgagggt tcaagtccct ctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.69e-08
primerB	100.0	20 bp	0	0	598	579	2.69e-08

Match details