JRC GMO-Amplicons | EURL GMFF

Record ID:	AF327594.1
Database:	nt	Go to original record
Description:	Quercus petraea tRNA-Leu (trnL) gene, intron; chloroplast gene for chloroplast product.
Taxon:	Quercus petraea
Original size:	558 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	558	+	558 bp

Amplicon:

558 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acttaccaag tgataacttt caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc ctattttacg aaaacaaata agggttcaga
151   agaaagcaag aataaaaaaa ggataggcgc agagactcaa tggaagctgt
201   tctaacaagt ggggttgact tctttacgtt attacattaa cgtaatcctt
251   atatcaaaac tacagaaagg ataaacctat atacatacgt atatgtactg
301   aaatcctatc tcaaatgatt aatgacgacc cgaatcttta tttatttata
351   tttctataaa taataaataa aaatcgaaag agttgttgtg aatcgatcca
401   aattgaagaa agaatcgaat atttattaat aaaatttatc gtacgagaat
451   aaagatagag tcccattcca cacgtcaata ccgacaagaa tgaaatttat
501   aggaagagga aaatccgtcg actttagaaa tcgtgagggt tcaagtccct
551   ctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.51e-08
primerB	100.0	20 bp	0	0	558	539	2.51e-08

Match details