JRC GMO-Amplicons | EURL GMFF

Record ID:	AF327592.1
Database:	nt	Go to original record
Description:	Pinus sylvestris tRNA-Leu (trnL) gene, intron; chloroplast gene for chloroplast product.
Taxon:	Pinus sylvestris
Original size:	553 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	553	+	553 bp

Amplicon:

553 bp

1     cgaaatcggt agacgctacg gacttgatcg aattgagcct tggtatggaa
51    acctaccaag tgatagcttc caaatccagg gaaccctggg atattttgaa
101   tgggtaatcc tgagccaaat ccggttcatg aagacaatgt ttcttctcct
151   aagataggaa ggggataggt gcagagactc aatggaagct attctaacga
201   atgaatctca tttggtccaa tactgtagtt atagaacgat ctatttacac
251   ctcaaaaatg gagagagatg ttagataaca tcagacaaaa ctggcgatca
301   gaactcgttc caagcatttt attcataaga tagatgccag attcgagttg
351   aagtactgat ttgacattaa gtaatccaat tatgaactta tctactctag
401   atggagaatt gaattatttt ttggaataaa tggttggacg aggataaaga
451   tagagtccaa ttctacatgt caatgtaaac aacaatgcaa attgcagtag
501   gaggaaaatc cgttggcttt atagaccgtg agggttcaag tccctctatc
551   ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.49e-08
primerB	100.0	20 bp	0	0	553	534	2.49e-08

Match details