Match details
Record ID: |
AF327589.1 |
Database: |
nt |
Go to original record |
Description: |
Pseudotsuga menziesii tRNA-Leu (trnL) gene, intron; chloroplast gene for chloroplast product. |
Taxon: |
Pseudotsuga menziesii |
Original size: |
550 bp |
View other 20492 matches for this method
Predicted amplification details: |
Mismatches |
Gaps |
From |
To |
Strand |
Amplicon size |
0 |
0 |
1 |
550 |
+ |
550 bp |
|
1 cgaaatcggt agacgctacg gacttgatcg aattgagcct tggtatggaa
51 acctaccaag tgatagcttc caaatacagg gaaccctggg atatattgaa
101 tgggtaatcc tgagccaaat ccggttcatg gagacaatgg tttcttctcc
151 taggatagga aggggatagg tgcagagact caatggaagc tattctaacg
201 aatgaatctc atttggtcca atactgtatt tatagaacgc tctatttaca
251 cctcgaaagt gagaatgtta tataaaacaa aactcgcgat cagaacttga
301 atccttccaa gcattttctt cgtaagatag atgccagatt cgagttgaag
351 taatgatttg acattaagta atccaattat gaacttatct actctagata
401 gagagtttaa tcagtttttg gaattaatgg ttggacgagg ataaagatag
451 agtccaattc tacgtgtcaa tgtcaacaac aatgcaaatt gcagtaggag
501 gaaaatccgt tggctttata gaccgtgagg gttcaagtcc ctctatcccc
Primer/Probe |
% Identity |
Length |
Mismatches |
Gaps |
From |
To |
Score |
primerA |
100.0 |
20 bp |
0 |
0 |
1 |
20 |
2.47e-08 |
primerB |
100.0 |
20 bp |
0 |
0 |
550 |
531 |
2.47e-08 |
|
Related publications:
Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101.
Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.