JRC GMO-Amplicons | EURL GMFF

Record ID:	AF327584.1
Database:	nt	Go to original record
Description:	Larix decidua tRNA-Leu (trnL) gene, intron; chloroplast gene for chloroplast product.
Taxon:	Larix decidua
Original size:	550 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	550	+	550 bp

Amplicon:

550 bp

1     cgaaatcggt agacgctacg gacttgatcg aattgagcct tggtatggaa
51    acctaccaag tgatagcttc caaatccagg gaaccctggg atatattgaa
101   tgggtaatcc tgagccaaat ccggttcatg gagacaatgg tttcttctcc
151   taggatagga aggggatagg tgcagagact caatggaagc tattctaacg
201   aatgaatctc atttggtcca atactgtatt tatagaacgc tctatttaca
251   cctcgaaagt gggaatgtta tataacacaa aactcgcgat cagaacttga
301   atccttccaa gcattttctt cgtaagatag atgccagatt cgagttgaag
351   taatgatttt acattaagta atccaattat gaacttatct actctagata
401   gagagttgaa tcagtttttg gaattaatgg ttggacgagg ataaagatag
451   agtccaattc tacgtgtcaa tgtcaacaac aatgcaaatt gcagtaggag
501   gaaaatccgt tggctttata gaccgtgagg gttcaagtcc ctctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.47e-08
primerB	100.0	20 bp	0	0	550	531	2.47e-08

Match details