JRC GMO-Amplicons | EURL GMFF

Record ID:	AF327583.1
Database:	nt	Go to original record
Description:	Ginkgo biloba tRNA-Leu (trnL) gene, intron; chloroplast gene for chloroplast product.
Taxon:	Ginkgo biloba
Original size:	574 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	574	+	574 bp

Amplicon:

574 bp

1     cgaaatcggt agacgctacg gactcgatcg aattgagcct tggtatggaa
51    acctactaag tgatagcttt caaatccagg gaaccccggg atatttcgaa
101   tgggcaatcc tgagccaaat ccggtttacg gagacattat tctcccagga
151   agagaaggga taggtgcaga gactcgatgg aagctattct aacgaataaa
201   gatcgtttta cccagtactg tatctataga aaaatctctc catttacact
251   ttggaagtgg ggttggtata tactaccaaa aaagatcatg atcaggactt
301   ggattggatc attttatgca tttcactatg catttcacta ttagtaaggt
351   aagatgcttg ggtcaatccc aagttgaagg aattatttta cattaagtaa
401   tccaattctg aactacccta aagagggagt cggatgaagt ttgggaagaa
451   atgatcggac gaggataaag atatagtcca attctacacg tcaatgccaa
501   caacaatgcg aattgcagta agaggaaaat ccgtcggctt tatagaccgt
551   gagggttcaa gtccctctat cccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.58e-08
primerB	100.0	20 bp	0	0	574	555	2.58e-08

Match details